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The the UVVisNIR spectra of TMB was measured on a PerkinElmer Lambda 750 UVVisNIR spectrophotometer

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Captured mAb variants were detected by an APconjugated polyclonal anti-human IgG Fc specific Ab from goat (Sigma-Aldrich, A9544) (diluted 1:5000 in PBS/T/S) and visualized by addition of p-nitrophenylphosphate (Sigma-Aldrich) (10 g/mL in diethanolamine buffer)

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10.1038/s41598-017-04409-x CrossRef Google Scholar View reference in article 12 BrattwallC