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The addition of 2 times loading buffer (62.5 mM TrisHCl, pH 6.8, 25% glycerol, 2% SDS, and 0.01% Bromophenol blue, and 250 mM dithiothreitol) solutions to the serum was performed in a 1:1 ratio

TA is classified according to the presence or absence of VSD or TGA

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Treatment with Sativex improved a number of outcomes in both patient groups, but crucially, it markedly and significantly decreased the self-rated pain scores and improved the quality of life in MS patients with neuropathic pain