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1 g of peptides were separated on a 15 cm length 75 m ID C18 separation column (Pepmap Acclaim column, ThermoFisher) using a linear 45-minute acetonitrile gradient

Activation of amylin receptors triggers signalling pathways that promote satiety and reduce food intake, partly by enhancing feelings of fullness and reducing hedonic eating drive

Ethics declarations Ethics approval and consent to participate This study was conducted in accordance with the ethical principles of the Declaration of Helsinki

Users experiencing these symptoms should immediately discontinue problematic product, apply gentle fragrance-free moisturizer for barrier support, and consult dermatologist or ophthalmologist if symptoms persist beyond 48 hours after stopping as outlined in peptide safety protocols