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We performed all the work reported here at an external pH equal to the cytoplasmic pH since, under these conditions, the weak acids equilibrate between the internal and external medium but do not concentrate driven by pH gradients 13

Keywords: oxidative stress, neurodegenerative diseases, hyperexcitability, motor neuron 1

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The studies to date illustrate that the continued development of protocols for harnessing and interpreting the label free, high content spectral information which Raman microspectroscopic analysis can yield is imperative, and this is further driven by the demands of emerging technologies