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doi: 10.1186/gb-2009-10-11-r130

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2 2.8.3 Widely targeted metabolomics analysis Frozen tissue specimens were homogenized and extracted using ice-cold methanol:water (7:3, v/v) supplemented with isotope-labeled internal standards,followed by multi-platform LC-MS/MS analysis

Terrible we have to beg for treatment
The solution is not ruined
