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Complex pathologic roles of RIPK1 and RIPK3: moving beyond necroptosis

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The reaction time was defined by placing the mouse on the hot plate, and the time was recorded when the animal showed hind paw licking or jumping, and the 45 sec period was observed to avoid tissue damage

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Purified Flag-GCLC or recombinant GST-GCLC protein was incubated with a gradient concentration of succinyl-CoA in succinylation buffer, followed by western blotting analysis using an antibody against succinylated lysine (K)