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Studies have shown that muscle RING-type E3 ubiquitin ligase 1 (MuRF1, TRIM63) and muscle atrophy F-box protein (MaFbx) in the ubiquitinproteasome pathway are closely related to the degradation of muscle protein in cachexia patients [15,16,17]
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After washing with PBS, tissue sections were incubated for 1 hr with secondary antibody: anti-mouse-IgG Alexa fluor 488 for cPT1/cPT2 and anti-rabbit-IgG Alexa fluor 594 for neurofilaments
Im not proud of it, but I have used sunbeds, says the 29-year-old graphic designer from Plymouth
