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10.1016/s0014-5793(00)01159-5 FEBS Lett

To remove debris, astrocytes were harvested by trypsinization, and the lysed astrocytes were centrifuged at 12,000 g for 10 min

Autophagy is identified by the accumulation of acidic vesicular organelles

10.1016/j.devcel.2010.12.003 Dev Cell

They are important for the proper functioning of the adrenal glands