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Bacterial colonization assay Animals were treated under behavioral assay condition with indicated bacterial mutants for 12 h, picked into tubes with M9 buffers, washed three times, and placed on empty NGM plates containing 100 g/mL carbenicillin for 1 hour
1c and d) and the lower body weight was maintained until the end of the experiment
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Frozen sectioned slides were subjected to immunofluorescence (IF) to confirm the distribution and determine the level of GSK-3 protein in the brain
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