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that is, respective 50%, 31%, and 19% comparisons showed lower, equal, and higher levels in vitiligo samples

Peters S, Koh J, Choi DW

Briefly, 15L of cDNA from the 10X GEM-RT reaction was input to a PCR reaction (TAP PCR 1) containing a CROPseq-RFP specific forward primer binding 54 bp upstream of the 3 gRNA sequence (CROPouter) and a reverse primer binding the Illumina Truseq Read 1 (Partial Read 1), producing an amplicon approximately 550-600 bp long covering the gRNA sequence and retaining the 3 10X cell barcode and UMI sequences

Activation of the UPR generally serves as a readout for ER stress, but it is not clear what diminished ER stress implies in terms of an actual accumulation of unfolded proteins, particularly because some stressors, such as palmitate loading, activate the UPR apparently independent of the protein folding process (Volmer et al., 2013)

Helped perform the analysis and engaged in constructive discussions: Hongchao Tang