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Given the direct nature of the PDZ domain of SNX27 in binding to VPS26A and VPS26B and the importance of this coupling for Retromer-mediated cargo sorting 11 , the lack of significantly enriched labelling may result from a technical limitation or reflect a highly dynamic, transient association between cargo-loaded SNX27 and the Retromer demarcated sub-domain during the handover of captured cargo 31

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