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After being fed HFD for a total of 16-weeks (an appropriate rodent model of DIO and comparable to human obesity [21]) and reaching pre-arrival body weights (~38g), mice were randomized into balanced control and treatment cohorts (n=9/cohort), with similar group average body weight and standard deviation

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By preventing NNMT from draining the nicotinamide pool, 5-amino-1mq helps maintain NAD+ at levels that support optimal energy production

This is a Molina-specific cap that differs from the CMS LCD in both threshold and code list and should not be conflated with the A56681 limits