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Cell pellets were collected and resuspended in lysis buffer (50 mM Tris pH 8.0, 200 mM NaCl, 5 mM -mercaptoethanol, 10% glycerol, and 1X Halt protease inhibitor (Thermo Fisher), and frozen overnight at 80 C

doi: 10.1073/pnas.2303077120

It helps regulate cellular growth and apoptosis

As the quality of kefir can be driven by factors such as fermentation temperature, time, types of kefir grains, substrate availability, more research studies on kefir production are needed to further validate and determine the exact parameters for producing a consistent and standard kefir

and blocking c-Junmediated transcription of innate immune factors [128]