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KPV (Lys-Pro-Val)# Evidence Level: Preclinical (animal studies + in vitro) Research Status: Not FDA-approved

The membrane was then incubated with appropriate horseradish peroxidase-conjugated secondary antibodies (1:5000)

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C., Couldwell, W

In intestinal Caco-2 cells, IL-1 decreases TER and increases inulin flux, which is in part mediated by the decreased expression and redistribution of occludin and increased expression of MLCK and MLC phosphorylation.46 It was also shown that the knockdown of nuclear factor-B p65 inhibits the decrease in occludin and increases in MLCK expression, indicating nuclear factor-B-dependent transcriptional regulation by IL-1.47 4