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After 24 h of migration or invasion in the medium (Boster) with 10% fetal bovine serum, cells at the bottom of the insert were fixed with 4% paraformaldehyde (PFA) for 10 min, washed three with PBS, and stained with 0.5% crystal violet for 0.5 h

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Weekly dose Units per injection (mL) Syringe fill level Frequency: Once-daily subcutaneous administration

In such cases, SOD mimics are supplemented with catalase mimics to alleviate vascular complications caused by hydrogen peroxide-induced oxidative damage (Prasad et al

The slides were subsequently incubated with anti-GCLC and anti-GSS antibodies (1:1,000) at 37C for 32 min, and 3,3-diaminobenzidine was used as a chromogen