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After the respective treatments, the cells were detached from the culture surface, mixed with 0.75% low-melting agarose (LMA) and dropped gently onto a microscope slide precoated with a layer of 0.75% normal-melting agarose

J Neurooncol 100:165176

D'Onofrio N, Servillo L, Giovane A, Casale R, Vitiello M, Marfella R, Paolisso G, and Balestrieri ML

metM-Wnt lung cells proved more resistant than either E0771 or M-Wnt cells to RSL3 (Fig