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These innovations are supported by rigorous evaluations confirming their safety, viability, and therapeutic efficacy in both adult and pediatric populations ( 5 Cellular, molecular, and systemic mechanisms of probiotics Recent advances in probiotic research have substantially refined the selection, genomic characterization, and functional assessment of specific microbial strains, leading to a deeper understanding of their mechanisms of action and their measurable impacts on human health ( Key mechanisms through which probiotics exert beneficial effects include the reinforcement of the intestinal epithelial barrier by stimulating mucin protein synthesis and regulating tight junction proteins such as occludin and claudin-1 ( In the GI environment, probiotics counteract harmful microorganisms by shortening GI transit time, limiting opportunities for pathogen colonization, and stimulating the production of bioactive metabolites, such as short-chain fatty acids (SCFAs), which lower colonic pH

[DOI] [PMC free article] [PubMed] [Google Scholar] 7.Andreadou I., Schulz R., Papapetropoulos A., Turan B., Ytrehus K., Ferdinandy P., Daiber A., Di Lisa F

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