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The column was initially hydrated with 0.5% acetonitrile and 99.5% of the phosphate buffer (NaH 2 PO 4 ) at a linear rate (Table 2) from 0.4 mL/min to 0.6 mL/min over 4 min, increased to 2% acetonitrile for 0.5 min at 1.25 mL/min and then to at 1.5 mL/min over 1.5 min, then changed linearly to 65% acetonitrile over 0.9 min and finally returned to 0.5% acetonitrile and 99.5% of the phosphate buffer at 1.5 mL/min for 2 min equilibration
Moreover, based on commonalities in their receptor chains and sequences and other functional characteristics, ILs can be further categorized into families

J Hepatol 2021;75:454-461

Because of the increased risk of food-bound vitamin B 12 malabsorption in older adults, the FNB recommended that adults over 50 years of age get most of the RDA from fortified food or vitamin B 12 -containing supplements (21)
