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coluzzii (UAS- AncCoeae6g ) Coeae6g coding regions under UAS control and marked with YFP (under the 3 P3 promoter), were established by integrase-mediated transgene exchange into the genomic sites of docking lines Ubi-GAL4 41 and A11 (previously described in Lynd et al.) 42 , respectively, as depicted in Supplementary Fig

The RT-PCR mixture contained 5 L 5xQIAGEN OneStep RT-PCR Buffer, 1 L dNTP Mix, 1.5 L of 10 M sense primer, 1.5 L of 10 M antisense primer, 1 L OneStep RT-PCR Enzyme Mix, RNA as described and RNase-free water

12 Although these compounds are beneficial in the free form, it was also confirmed that when bound to lens proteins, as occurs with increasing age in the lens nucleus, these UV filters become stronger photosensitizers which can, in the presence of oxygen and UV radiation, generate singlet oxygen, superoxide anion, and hydrogen peroxide within the lens nucleus 24 Hydrogen peroxide, although producing little direct damage, can be converted into highly reactive and damaging species in the presence of transition metals