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The receptor is specific to the IF-B 12 complex, and so will not bind to any vitamin content that is not bound to IF

The neurons of the cortical cerebral, cerebellar region, hippocampus, and hypothalamus were counted in 10 different high-powered fields (HPF, 400x) and 3 to 5 serial sections of each sample were used to do the count as described in The field size was 0.24 m 2

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If you order your B12 shots elsewhere, make sure they come from a certified lab that follows stringent pharmacy regulations

The BCA Protein Assay Kit (P0012, Beyotime, China) was used to quantify protein concentration