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To create the ges-1p::cpt-4 , we ligated a 2 kb ges-1 promoter to the coding region of cpt-4 (cloned from N2 genomic DNA) and unc-54 3UTR using Gibson Assembly
For this experiment, single D1 wild-type or mutant males were mated to a single L4 wild-type hermaphrodite for 48 hrs and then removed
Republican," Paul was a businessman, patriot, public servant, politician, and -- most proudly -- a family man
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