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In studies of GPX5 knockout mice, we deduced that GPX5 is a way to maintain the integrity of sperm DNA function by removing excess H 2 O 2 , and these mice did not exhibit significant defects in the epididymis and sperm cells ( 2 O 2 scavengers was detected (Noblanc et al., 2012), proving that after SNGPX4 and GPX5 double knockouts, the epididymal tissues of mice produced oxidative stress responses, indicating that SNGPX4 and GPX5 play a role in maintaining the normal structure of the sperm nucleus in the epididymal tissue (Noblanc et al., 2012)

Microinjection was performed using the following injection mix: KCl (25 mmol L 1 ), Hepes pH 7.4 (7.5 mmol L 1 ), tracrRNA (200 ng L 1 ), dpy-10 crRNA (150 ng L 1 ), dpy-10 ssODN (13.75 ng L 1 ), adh-5 sgRNA (300 ng L 1 ), adh-5 ssODN (100 ng L 1 ), Cas9 (416 ng L 1 , NEB, USA) 59

Vitamin B6 (Pyridoxine) : Essential for protein metabolism and cognitive development

Berberine is a dopamine D1- and D2-like receptor antagonist and ameliorates experimentally induced colitis by suppressing innate and adaptive immune responses