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Importantly, despite the dramatic decrease in the level of labeled M3 C3 in healthy and MMA hepatocytes treated with BCATi (Figure 3D), treatment with the inhibitor had no significant effect on the concentration of the unlabeled pool of C3 (M0 C3, Figure 3E) or the concentration of the total pool of C3 (M3 C3 + M0 C3 = total C3, Figure 3F) in healthy or MMA hepatocytes exposed to [ 15 N, 13 C 5 ]valine