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Importantly, animals were singly housed to eliminate the exchange and cross-contamination of intestinal microbiomes, a factor that potentially biases studies with group-housed animals by minimizing microbiome variations for co-housed animals within a cohort
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The magnitude of the NAD + -elevation effect of NNMT inhibition in vivo, and its contribution to the metabolic phenotype relative to the methylation effects, remains an active area of research
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