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For the purposes of this exercise, lets suppose that youre starting with 100 mg of GST-fusion protein from one liter of fermented culture

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Your choice of binding buffer will influence the binding capacity of the beads, so if you are capturing substantially less than 8 mg of protein per mL of resin, and your protein isnt really big, then it is worth considering if your binding buffer is limiting the capture efficiency of the beads

It supports the normal function of nerve cells and is necessary for red blood cell formation and DNA synthesis

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