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Storage of blood samples prior to separation of the serum affects B12 concentrations if separation is delayed

Subsequently, 0.5 mL of reagent 2 was added to the mixture, and incubated for another 30 min

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The incubated lysate was centrifuged at 6,000 g for 10 min at 4 C, filtered through a 5-m PVDF filter (Millipore, SLSV025LS) and then added to the prepared beads and incubated at 25 C for 1 h with end-over-end rotation
