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A possible role for mitochondrial dysfunction in migraine

24 , 614623.e616 (2017)

Ethics statement The animal study was approved by the Institutional Animal Care and Use Committee at Cairo University, Cairo, Egypt (Vet CU13102024983)

Then, the cells were lysed on ice in 800 l of lysis buffer 1, which was composed of 7 M urea (J.T.Baker), 30 mM tris(hydroxymethyl)aminomethane (Sigma-Aldrich), 2 M thiourea (Sigma-Aldrich), 0.1% Triton X-100 (Sigma-Aldrich), protease inhibitors (EDTA free cocktail tablet, Roche Diagnostics, added according to the manufacturers instructions) and phosphatase inhibitors [1 mM sodium orthovanadate (Sigma-Aldrich), 9.5 mM sodium fluoride (J.T.Baker) and 1 M okadaic acid (Sigma-Aldrich)]

Cholinesterase inhibits the breakdown of acetylcholine, a chemical messenger critical for learning and memory