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24 h after the treatment, cells were fixed with 10% trichloroacetic acid, extensively washed, then incubated with the dye, washed and finally resolubilized in 10 mM Tris base, prior to scanning the plates at 565 and 690 nm (main signal and background, respectively)

Marasinghe et al., 2005)

The ability of plant GLRs to respond to such a broad spectrum of ligands is closely linked to their conserved yet flexible domain architecture, as illustrated in Fig

Quick spin and collect the supernatant

[DOI] [PMC free article] [PubMed] [Google Scholar] 114.Rayhan R.U., Stevens B.W., Raksit M.P., Ripple J.A., Timbol C.R., Adewuyi O., VanMeter J.W., Baraniuk J.N