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Animals were anesthetized and maintained using isoflurane (1-2% in O 2 ) and placed head first in the prone position
doi:10.1073/pnas.0701096104 Zhang C, Rodriguez C, Spaulding J et al (2012) Age-dependent and tissue-related glutathione redox status in a mouse model of Alzheimers disease

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This strategy will help shorten the time required for treatment development and will reduce costs 35

New crystal Structures of Human Glutathione Transferase A1-1 Shed Light on Glutathione Binding and the Conformation of the C-Terminal helix