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Furthermore, they found that this transformation can enhance the degradation rate of HSA, but may disrupt the ligand binding capacity of Cys-487 and nearby sites

doi: 10.1111/jcmm.16581 29 ZhangHWangAShenGWangXLiuGYangFet al

The sample was finally dialyzed to remove all chaotropic agents and redox reagents and end the refolding process

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In obese adipose tissue, excessive ROS production from NADPH oxidases and impaired ETCs induces mitochondrial DNA (mtDNA) damage, which escapes into the cytoplasm and circulation as damageassociated molecular patterns (DAMPs), activating TLR9 and NLRP3 inflammasome pathways to trigger proinflammatory cytokine cascades such as IL1 and IL18 [118, 119]