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The membrane was blocked with 5% BSA for 60 min at room temperature, and then incubated overnight at 4 C with the following primary antibodies: anti-sirt1 (1:1000, CST), anti-AMPK (1:1000, CST), anti-p-AMPK (1:1000, CST), and anti-GAPDH (1:1000, CST)

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10.1021/acs.jafc.8b03298 J

That just means, one, which I didnt read that one study, but there is one study that talks about mutating your genes, but thats a little different than what Brendan was talking about, but could cause cancer as well