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Fluorescence microscopy For super-resolution microscopy of live cells, U-2 OS FSP1 GFP-P2A-BFP knock-in cells were seeded in 35-mm imaging dishes (CellVis, D35-14-1.5-N) 48 h before the start of imaging

In contrast, mutation of the C-terminal active site cysteine increases the activity of GLRX, presumably by preventing disulfide bond formation between the two active site cysteines 20,21 , and renders GLRX a monothiol enzyme only capable of catalysis of (de)glutathionylation 22

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The reaction was halted by the addition of sulfosalicylic acid and protein precipitate was removed by centrifugation

2020;194 doi: 10.1016/j.colsurfb.2020.111193