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Even though metabolic flux assays provide more information than global metabolic profiles, the limitation to these methods is the time window available to harvest the tissue and cells of interest without losing the cellular tracing

If the vitamin status is normal, then the values of the basal and stimulated activity are similar, and the EGR activity coefficient is very close to 1 Measurement of EGR AC has been used to monitor riboflavin status only in a few population-based studies such as the UK National Dietary and Nutrition Surveys Rolling Programme which includes all age and sex population groups (Bates et al., 2016), Irish National Adult Nutrition Survey (Kehoe et al., 2018) and the older national surveys from Germany (Heseker et al., 1992) and the Netherlands (Lwik et al., 1994)