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In the mitochondria, by the activity of the pyruvate dehydrogenase, the glycolytic pyruvate is converted in acetyl-CoA which represents a negative modulator of the pyruvate dehydrogenase

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16s rRNA sequencing on fecal pellets DNA from fecal pellets was extracted using a Quick -DNA Fecal/Soil Microbe DNA Miniprep Kit (Zymo Research, #D6010) according to the manufacturers instructions

Here they showed that feeding the mice with a probiotic of Akkermansia , led to reversed effects of high-fat diet (HFD) and improved glucose tolerance
