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Another study revealed that pravastatin, an inhibitor of cholesterol biosynthesis, induced Mdm2 Ser166 phosphorylation, which increased Mdm2 ubiquitin ligase activity, attenuated p53 degradation and inhibited the DDR in hepatocytes 118

A 40 L aliquot was removed and quenched with 6x SDS sample buffer as an input control

Sod2 +/- mice crossed with Apoe -/- mice have increased atherosclerosis compared to control Apoe -/- mice (410), but there is little effect on atherosclerosis of crossing Sod3 -/- mice with Apoe -/- mice (411)

In these transgenic tobacco plants, the expression of genes PALs , CHS , CHI , F3H , F3H , ANS , and DFR (Table 3, Figure 4) was significantly higher compared to control tobacco plants

Nrf2 nuclear binding was assessed by using an ELISA based Nrf2 TransAM transactivation kit (Active Motif, Carlsbad, Calif) according to manufacturers instructions