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Besides, TMAO is excreted mainly through the kidney, 96% of which is eliminated in urine by 24 h (Taesuwan et al., 2017)

Chain-breaking antioxidants such as vitamins E and C halt the process of radical formation by stabilizing free radical molecules so that the chain-like process of radical formation is arrested

These are two distinct classes acting through different receptors

Intraperitoneal injection of male albino rats with 200 mg acetaldehyde/kg body weight, daily, for 10 days, with additional pyrazole treatment to inhibit the conversion of acetaldehyde to ethanol, caused fatty accumulation in the liver, as indicated by accumulation of total lipids, triacyl glycerols, and total cholesterol, increased glycogenolysis, and a shift in metabolism from the citric acid cycle towards the pentose phosphate pathway in the liver

The final Illumina libraries were bead-purified, pooled and sequenced on NovaSeq 6000 instrument (Illumina) following a 100-bp, paired-end recipe