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CaCo-2 (human colorectal adenocarcinoma cells) and Huh7.5 cells (human hepatocellular carcinoma cells) were maintained at 37 C with 5% CO 2 in DMEM containing 10% FCS, GlutaMax, 1% ( v/v ) nonessential amino acids and 100 g/mL penicillin-streptomycin

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doi:10.1096/fasebj.22.1_supplement.445.8

So, antioxidants extra t efonct in biological ssystemswith the help of various mechanisms such as gene expression regulation, co-antioxidants, metal ion chelation, and electron donation (Lobo et al., 2010)