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Bicuculline is a competitive antagonist of GABAARs primarily on the ionotropic GABAARs, ligand-gated ion channels, and the passing of chloride ions across the cell membrane [230]

Globally, 6.1 million PD patients were recorded in 2016, and the age-standardized prevalence rate (ASR) rose by 21.7% between 1990 and 2016 (Global, 2018)

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These thought-provoking questions will inspire studies that explore and discover new factors and functions of the classical pathway

In brief, the sections were incubated for 90 min at 25 C in blocking solution (10% horse serum in PBS), and then overnight at 4 C with primary antibodies: mouse anti-glial fibrillary acidic protein (GFAP) antibody (1:500, cat# MABN92, Sigma-Aldrich, USA) or mouse anti-ionized calcium binding adaptor molecule 1 (Iba1) antibody (1:500, cat# MABN92, Sigma-Aldrich, USA)