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Allow full absorption
Wingler K, Bocher M, Flohe L, Kollmus H, Brigelius-Flohe R
Micrographs are representative images of three independent experiments
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Cells were washed with regular PBS (pH = 7.2, lanes 1, 2, 3, and 5) or high pH PBS (pH = 10.75, lanes 4 and 6) to remove the surface-bound virus before detection of cell-bound L1 by non-reducing SDS-PAGE and western blot