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All the studied mutants show the same spectroscopic pattern as the WT, with the formation of a 440-nm absorbing species upon acidification to pH 2 (the effect is known as acid trapping and represents trapping of all- trans - retinal chromophore bound to opsin by means of a protonated Schiff base linkage in the denatured protein)

Introduction Proteins are major structural and functional polymers in living systems Proteins have a broad range of activities, including catalysis of metabolic reactions and transport of vitamins, minerals, oxygen, and fuels