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The importance of proteins in trafficking free cytosolic cholesterol to the mitochondria has been shown in mouse models, in which absent/inactive HSL results in increased adrenal accumulation of intracellular cholesterol along with impaired corticosterone production.(4,20) It appears that under physiologic conditions, the supply of cholesterol is not rate limiting, since multiple sources are available to provide ample cholesterol for steroid biosynthesis
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Prior to these treatments, each compound underwent dose-response assays using serial dilutions to determine the highest concentration that was both nontoxic and effective

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