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Serotonin is acetylated by aralkylamine N -acetyltransferase (AANAT) forming N -acetyl serotonin
Cells were harvested and lysed in lysis buffer (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 10 mM imidazole, and 2 mM PMSF) using a Pulse 150 Ultrasonic Homogenizer with 6 probe (Benchmark Scientific, Sayreville, NJ)
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Prostaglandins Other Lipid Mediators 148 , 106411 (2020)
