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p21 positive cells detected by immunofluorescence method in mouse skin Paraffin-embedded mouse skins were immunolabelled with antibody to p21 (rat anti-mouse from AbCam), and immunofluorescence performed as we describe previously 64

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By compromising mitochondrial turnover and quality in response to cold stress, defects in both mitophagy and mitobiogenesis brought on by the loss of FUNDC1 raise the possibility that maintaining the proper balance between these processes is essential in meeting the increased demand for mitochondrial function that arises in some tissues under diverse environmental pressures [230]