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Antioxidants (Basel) , 8, 119, (2019)
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Cryo-EM grid vitrification and data collection For GluK3 D759G UBP310+spermine , prior to vitrification, 0.5 mM UBP310 and 1 mM spermine were added to the purified protein and incubated for 20 min on ice to trap the GluK3 (D759G) receptor in a closed state
