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We have a family of enzymes who use this antioxidant pipeline to generate superoxide called NADPH Oxidases (NOX)

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For differentiation, one day post-passaging, HM was replaced with expansion medium (EM), composed of advanced DMEM/F12 with 1% P/S, 1 mM GlutaMax, 1 mM HEPES, 1% N-2, 2% B-27 without vitamin A, 50 ng/mL EGF, 25 ng/mL HGF, 100 ng/mL FGF10, 1mg/mL R-spondin 1, 25 ng/mL BMP7 (PeproTech), 5 M A83-01, 10 M forskolin, 1mM N-acetyl L-cysteine, 10 mM nicotinamide, and 10 nM Leu15-gastrin I

doi: 10.1016/S0021-9258(20)82128-0 48 DeBerardinisRJMancusoADaikhinENissimIYudkoffMWehrliSet al

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