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Barcode plasmid pool preparation To generate the yeast CloneSelect barcode library, a semi-random oligonucleotide pool, KI#200, encoding 5-CCGWSNSWSNSWSNSWSNSNGTG-3, was chemically synthesized (Supplementary Table 2) and amplified by PCR in a 40 l reaction containing 2 l of 0.01 M template, 2 l each of 10 M forward primer SI#368 and 10 M reverse primer SI#369, 0.8 l of Phusion High-fidelity DNA Polymerase (NEB, no

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Sterile Syringes: We recommend using a larger syringe (3mL or 5mL) for the initial mixing to have better control, and standard 1mL U-100 insulin syringes for measuring small amounts during research handling

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The goal is to develop safe and effective treatments to ease the burden of this condition