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Based on this the use of phosphate buffer, pH 7.4, was suggested by Silk et al to reduce the undesirable non-enzymic hydrolysis to negligible proportions despite of a pH optimum of 8.5 26

10.1152/physrev.00023.2017 [DOI] [PMC free article] [PubMed] [Google Scholar] 331.Torzewski J, Heigl F, Zimmermann O, Wagner F, Schumann C, Hettich R, et al

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This heavy isotopologue may be used as internal standard and e.g