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When Piezo1 expression is upregulated, past experiments have shown that small injections of the Piezo1 channel agonist Yoda1 into the BF of mice produce the same pathological manifestations as those of sleep deprivation (Ma et al., 2022)

I would respectfully ask if the B12 test could be repeated, I have learned here that B12 supplementing can alter folate levels so I wonder if your folate level being considerably above the high end of the range has masked your B12 deficiency and affected your B12 levels

Most of the available literature comes from early research conducted in the 1970s and 1980s and from preclinical work, while the contemporary, rigorous literature is relatively sparse

Sleep Disruption Irritability Worsening Behavior Aggression These side effects often improve with dosage adjustments or timing changes