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doi: 10.1038/s41467-020-17181-w 55 ZhiweiYTaiyanYXuWKelinMTianlaiWBoyuWet al

Cells were treated with 30 nM xCT siRNA (Ambion) for 24 h followed by replacement with serum free DMEM/F12 media for additional 12 h

It also needs to be neutralized to actually thicken, and because it is a large molecule, it doesn't really penetrate the skin barrier

doi: 10.1152/physrev.00003.2008

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