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For calcium imaging experiments, OTCs were transduced with the genetically encoded calcium indicator jRCaMP1b 30 via bulk loading of 0.7 l of AAV9-Syn-NES-JRCaMP1b (Addgene #100851), diluted in PBS to a titer of 4 10 12 vg/ml

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These investigations are essential in expanding scientific knowledge about peptide-based mechanisms related to cellular longevity, skin hydration, and follicular regeneration